CRIMIC CR00389
CG8733
Information
PCR Verification
PCR check relative to sgRNA sequence
| HA-L |
HA-R |
Forward |
Reverse |
| short band |
OK |
TTGGAATTGTCGAGAAGAGCGGTCG |
GTGCTCCTCGATGGTGTCCATAAAGA |
Images
Construct Design Sequences
| sgRNA sequence |
ATCAGCCCGGCCCTTGTTACTGG |
| sgRNA2 sequence |
|
| Amplicon PCR 5' Left |
Amplicon PCR 5' Right |
Amplicon PCR 3' Left |
Amplicon PCR 3' Right |
| TCGCAGACGCAGAAAGAAGT |
CAAGGGCCGGGCTGATTTAT |
CAGACCAGTTAGGCCAGGTG |
CTTGGACGTCGGTCTCGTTT |
| Amplicon 5' |
Amplicon 3' |
| TCGCAGACGCAGAAAGAAGTTATCTGGTCGCCCCTCGAACACCTCCTGCAGCTGCACTTCCTTCACCATCTCGTGGCCAAGGGCAACCACCACATATTCGCGGCCCAGCTTCAGACCCACCAGGTCGCTCCGGAAGTCTTTTGCCCAGCGCTCGAACAGGATGTGCTGGCCGCCAACAGCACTAGCTTCCTTACGGAACTGGTAATATAATAAAGGAACGAAGGATTAATAACCCTAGTATATTTAATGATTTTGAGAATATATAGGTTTTTGAGATCTTGATGAACATATTCAGCTTGCCCTTTTCGACGAAACGTTATTTAAGATTTGGATTTCATTAAAGAACAATCTAGATTTTGGATGTTTCAATTATCTGTTTCGTATTCAAATAATCTCATTTTAATAGTATTTAAGAAACTTTTGTGAGCCTTACCTGTAATGTGTTTCCTAACCATGGCACAAATCGAGGTCCTGTTTTAGGAATGAAATTCAAAAATGATACTAATTTTAAATATTTGTCTTGTTAGCAATAGAATTTCATATGGAATACAACCGTCCAATTCAATCAATTCAACCTATGCATAGGTATCAAAGTGCTAATCTAATCGCCTGCAAAAGAAATCTGTTAGCAAGCAAAACTACCGAAACGAAAGTGAAAACTCTATCTTGAAATCAAACCGCTTAGTTATTAACTATCAATGCGTTATCAAAATCAATTTCATAACTTTTATATGTGGTCAAATTGAGACCGCTTTTCTTTAGGTGAATAAGATTCCCTTAGAAATTGCATACTAATTTATCACCATTTTGAACTGCAACAATTGCGAATAATAATAAAAATGTGCTTATATACAGGAATGACAGCTATTACGCCCATAATCGTAATCCTAATGCAAATCAAAAGCTGCATTGGTAAGTGGAATAAATCAGCCCGGCCCTTG |
CAGACCAGTTAGGCCAGGTGTCAGAAGTATGTGACGATTTTATCAATAAGATATATTAGCATTCAAAGCGTGTGCCGTCTGGTTCCCCGCCGATTGGCCAAAGATGCCTCTGATCACCATTACGAGTTGCTAATGAGCCCACCAATTAATTGGTCGCGGGGCAGGTCGTATGTTCGCCTATATACAAATATATTTTATAATAATATGGTTATTAATGCAAAGAATAATGCTTCTCTTCTATTAAATATAATTAATTGGCTAGTTTTCAAAAGCAAACTGTACCCACGATACACAAAATTGGTATTCTACGAATTTTAAAGCAAATCCATCGTTTGTTTGTGCACCAACGCTTTAACTTCTTATCTGCATTTGGGATTCCAGGAAAAGTTTTCCAAAGTGCAGCACGAGAAGAACTCACCTGGTGGAAAGTTCTTGGGACGCCGCGCCGAGCTGATGAGTGAATAAATCACGAAACCAATTAGAATCGCGCACAGAAATATGAGGGCACTCATTTCAATATACTATTTATAAGACCACCGAAAAACGAGACCGACGTCCAAG |
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