CRIMIC CR00531

Gene Vha100-1

CG1709

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p0	3R:29131914..29131932	98F12	FBti0200579	Order from Bellen Lab

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
no band	OK	CACGGTGCAATTATGGTCCGATCCAT	GATAGCGCCGATATTTGCTGGATGAG

Images

Construct Design Sequences

sgRNA sequence	GCGTTAAAGGGGACGTTTCATGG
sgRNA2 sequence

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
ACTTTCTTTTTGTCCGCGGC	TCGCTAATGAGGTAAGGGGT	ACGTCCCCTTTAACGCGAAT	GATATACGTGCCGAGTGGGG

Amplicon 5'	Amplicon 3'
ACTTTCTTTTTGTCCGCGGCGGAGAAAAATCAATATACGCATGGGGTATAGAAATCGAATCTCGGCGCATATATAGTATAGATTACAGACAAATAATCTGGGGGACAAGCGAACGTCCGCCCACCGAAACGATAACCAAATCAGCATGAAAATATGTATGCGACACGGCCGCAAAACTTAGGAAGAAACCCAGTTTGGCCATGAATCATGCAATTGAATGGGCCCATTGTGTACTATATATGTACATATGCATTCTGATTTAGTGTCTGTTCTCCATTCGCAGATATCGGGAATCCGGCTGCCACAATGGGTTCCCTATTCCGCAGCGAGGAAATGGCGCTGTGCCAGCTGTTCCTGCAGAGCGAGGCGGCCTACGCCTGCGTCTCCGAGTTGGGAGAACTGGGATTGGTCCAGTTCCGAGATGTGAGTAGGATGGGTTACATAAAGCTGCGGTCGAGGCAGCCGCACTGACTAACCATAAACCACAATCATACCCCTTACCTCATTAGCGA	ACGTCCCCTTTAACGCGAATGCAAAGTAAATGCAAAGCTGGTGACACCAGCTACTAAGTGTATAGTATAACTACGTGTGTTACCTCATTTAAATAAAATTGAAAAATATAGTGGCATAAGTGCTATATCTTGATCGCACAAATGAAGTATCTTTGAGATCCCCCAATAACAAACCAATCTTTTGAAATCCGCAGCTCAATCCCGATGTCAACGCTTTCCAGAGGAAGTTCGTCAACGAGGTGCGCCGCTGCGATGAGATGGAGCGCAAGCTGCGTTACCTGGAGAAGGAGATCAAGAAGGACGGCATCCCCATGTTGGACACCGGGGAGAGTCCCGAGGCCCCGCAGCCCCGCGAGATGATCGACCTGGAGGTATACGCCGCCATACTCCCTGTTTTCTGCTAAACACTGTTGAACTTATATGGCTTTCTTGTCTGCTGCTCAGGCCACCTTTGAGAAGCTGGAGAACGAGCTGAGGGAGGTGAATCAGAACGCCGAGGCCCTGAAGCGCAACTTTCTGGAGCTGACCGAGCTGAAGCATATTTTGCGCAAAACCCAAGTGTTCTTCGATGAGGTAAGCTGCGGCATGAGTAACTCCATATGGGTATCAGCTAACAAACTGCGATCGGTTACCGATTTACCATTAACAAATGCCGGCCATTGAAGCGGCCGGTAACTAACCAAATTTTCTAAGCAATAACCAATTTCCCAACCGACAACACAAGTGTGGCAGCCACGACTTCTAGTCACATAAGCAATCTATCCCAAATTACACTCTAAAATTATTTCCCCGTATCTCCCCACTCGGCACGTATATC

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