CRIMIC CR00667
CG9318
Information
PCR Verification
PCR check relative to sgRNA sequence
| HA-L |
HA-R |
Forward |
Reverse |
| no band |
OK |
ACCTTTATCTGCTCTTCGGGAAGTGC |
GTTCTCCAGTGGGTAGCACCATGTAA |
Images
Construct Design Sequences
| sgRNA sequence |
CGCAGTAACTAGTTATATGTGGG |
| sgRNA2 sequence |
|
| Amplicon PCR 5' Left |
Amplicon PCR 5' Right |
Amplicon PCR 3' Left |
Amplicon PCR 3' Right |
| GCTCCTCGCCCAAACAAAAG |
GTGGGGTCCATATGTGGCC |
TATCAGTGCTTCCGCCTGTG |
AGCTGTACTAGGTGGCAACG |
| Amplicon 5' |
Amplicon 3' |
| GCTCCTCGCCCAAACAAAAGTCGAAGTGATGGTACTCATAGGGTATTACGGATTCCTCAGTGTTTAAGCGGTTGACGTACAACAAGATTTCCGACTGAAGAAGATAATCAGGAATTAGATCAATCAATTGGATTGACAACTGTTGATAATACTAGTGTATTTTAATCTTATCGTGGAAATAATTATTAAAGTTTGGTAAAAATTCCAAAAGTTTGCAAATGCGTTCGATAATTTGACCCCGTAATTTGCTTTTACTTTGGGTTCTTAGAAATTTATCTTATCAAAAAGCTCTCTTAGATTGTTAGAAACAATTGAATGTGAAACACCTTAATTAATTCTTATCATTGGGCAACGTCTAATTTAAGGGCGTATAAACCGATTAGGATTTCTTGACCTACGGGTATATTAAAAAGTTGTTTAGATTTGTTTAAAGATAAGATGAAGCAAACAAAACCAATAGCTCACGCTTTATCATCCAATAGTTAGGTCATCTTTTAAAAGGGCTAACCCCATCTATTTGGGATATCAAACTAAAAATTATACTTTGTCCTGAATTTATATTGTTATTGTGTTATACTATTATTACTAAAAAGAAAATAACCCAAACATAATGCAAGGAAGCATGACAAGCTAACCGCGTTCCCTGGTTCAATTATTGGTTAGCCGCCACTATGTTAAGATTCTTAAGGTCGGGAGCCCCCGAAATCGCATTCGCGAGCCAGCTGAACCAACATTAGAACTCCCATCTAGCTGTCGCTAGGAAAGAGAGCTGGCGTAGGCAACGTCAGCAGAAGTTGAGATAGAGGGCCACATATGGACCCCAC |
TATCAGTGCTTCCGCCTGTGACCACCATTAGAGGAATATAGAACCAATGTACATAACTAGTTCCAGTGCCTTTGCTAATTGTGGCGGGCGTGGGGCAAACACACCCACTATCTACGTACATATGTACATCTGCACTTCTGCACATACGTATACACCTACCTTGCATGTGGATGAGACGTCGGTTTTCTTGCAGAAATTGACCGGTGCCAGTCCGGGCAGATAGAAGGCCTGTGTCGGGGTCGCAAGGTGCAAGAGAACGACGCCCAGGAGCGGCAGAAGTCCGGATAGCAGGATCATCTCGAATTGGGGTAACCAACGAACGGCAGGAGTAGCAGACCTTCTTCCTTTACTTGTACGGGTTGTAATCACAACGTGGCCGTGTCCGCGATTCTCTGCCACACTTTCCGGATCGGCGGGCCACGCACAAACCGTACTATCTGGATATTTATCGGAATATACGGAGTTTTTCGAGTGTTTTTACTACGTTTCCTCTTTTTTGCCGAACGTCAGCACTGTAGCAGCGTTGCCACCTAGTACAGCT |
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