CRIMIC CR00903
CG8735
Information
PCR Verification
PCR check relative to sgRNA sequence
| HA-L |
HA-R |
Forward |
Reverse |
| OK |
OK |
GCGAGGGTGGTAATGGATTTTCAACC |
AGTAATCATAACCCACCGTTGTGGGC |
Images
Construct Design Sequences
| sgRNA sequence |
ATTCGCTGTATACTACGTTCGGG |
| sgRNA2 sequence |
|
| Amplicon PCR 5' Left |
Amplicon PCR 5' Right |
Amplicon PCR 3' Left |
Amplicon PCR 3' Right |
| TGCTTCTTGTCGCCAAAACG |
GCGAATATACTAAGAGCCCCA |
TGGGCCGCTGCTTATACG |
GCGGAAGAAAGCGTAGTCCT |
| Amplicon 5' |
Amplicon 3' |
| TGCTTCTTGTCGCCAAAACGCTCCAGGAGATTCACCGCCACCTGGGAAATGCATAATGGTACATGATATAGTTTGAAGTATCTAAACAGAGTGCATATAAAAACAGGCGCGAACTCGACTTTGTGAAAGTCGATAAATAGCTTTTGCGTTCTAGTCTGATTTGCATTGATATGAGTTCTTTAAAACAGCTGTGCCCTTTCTATGGTTTACACTACGCATCTTAGCCGAAAATAAAGAGAATATTCTAAGCTCTGATAAGGACTCTGCACTCGGGCATGTAATCAAGGGGAAGTTGTGACCCCACCTTATAGGTCTCTTTGTCCATGACCTGCTCCAATATCTTGCGCTTGTCCTCCTTCAGACGAGTCAGCTTATCGCCATTTTTGTTGAGCTTGCGCTGGAAGTACCACGTAAACAGCCGCCGCAGAAAGATGATGCTAGAGCCCGGGCGAGAAAAAGGTGGGTCAGGAACCGGATTTAGAGCGGATTAGACGGCAGCACTCACACAATGGGGAATAGCAGCAGCGGTACCAGGTACATGAAGCACTCCTGCATGGTCGGCGGGAAGTAGAAGTACCACCAGAGCCCGAAGCCCACGATATATGCTCCGATGGTGAAGCCCACGAAGTTCGTCACGAACCGGCGCTTGCGCTCCTCGGTGTTGATCATGTACTTCTCCAACGCTTGGATCTGCGTCTGCAGCCCCTCCAACACCGCCTCCGTGGACTTTTCTTTCTATAATTAGATTCGCGAATTCACGACACATATTTTTTGGGGCTCTTAGTATATTCGC |
TGGGCCGCTGCTTATACGCACCCGCAGCTTGGATAACACAAATCCCATGGTGTATGATCCGCAATTTAACTAGAAACGAACAAACTTAGACATTTACTTGACTATTTTGCTATTTAACCGCCCGCTTGCCTAGATATTATTTGCGCTCAGCGTGACCGTGCCTGTACCTTTCGAATACACCGCGGGAACCCACCAAAAATACCAAATTCCTACAGTGCTATCGCGGACTCAAACCACGAGAGAATGTCCAATTGGAAATGTCCGCGTCTGTTCAAATTTCAAACTTTAAATATTTCATATTCTTATATGAAACCAATTTGAACGATTGATTATATTCATAATGGAACATTAAGTACTGAAGGTAAAACAAATTTACGAAGCATTGGGGCCTGAAGAAAATGAGTGTTAGAGTCGCCATTATTCACATGCCTTTATACTTAAAAACTATTTTTGTGGAACAATACGCCTTTTAACAAATTGTTGTTACTGTTAAGTTATCAACTATCTTTAAAGAGCCTACAAATATTTCAGCTCATTTCATTTGAAAATATTGCACTATCGTTAACAGAAGCGCATTGTATTTGGCCACCATCGAGCGATGGCCACACTGCCACTGCCTCGGCCACACTGCAGTTCGGATTTGCCGGTTGTTTGGATCGCAGATTGAATCGGAAAGCCAGCGGCGTTGGTTCGGAAAAGTGCAGTCTCCGCGTCGCGAGTGCGCCAATAACGAGAAATAACGAGAATTGAAAAAGTTCCTTAAGAGGTGGAGTCCAGTAAAGCGCAGCAGCAATGATGCCGCTGCAGGAGTCGCCGTCGCCCAGTTGGCAGCTGGAGGACTACGCTTTCTTCCGC |
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