CRIMIC CR01868
CG13950
Information
PCR Verification
PCR check relative to sgRNA sequence
| HA-L |
HA-R |
Forward |
Reverse |
| OK |
short band |
CTGGGAAGATGTCATTGGTTTGGACG |
TTGCATTCACAGTCAGACGGTGAAGC |
Images
Construct Design Sequences
| sgRNA sequence |
GACTTTAACCCAGATCAAAGCGG |
| sgRNA2 sequence |
|
| Amplicon PCR 5' Left |
Amplicon PCR 5' Right |
Amplicon PCR 3' Left |
Amplicon PCR 3' Right |
| AGGCCAAGATGGAGAACACG |
AGTGGAGAACCGGGTATCCA |
TCTGGGTTAAAGTCAAAGGTCA |
TCCGATCGAAGTTCACGTGG |
| Amplicon 5' |
Amplicon 3' |
| AGGCCAAGATGGAGAACACGGCTAGAAGACATTCTAGTAATAGTGGTTACTAGGAGTACTAAAATGTAGCAGTAAGATATAAAAAGGATATGAGCCACCATCAAGGAGTGCCTATTCCTCCAACTAGCACTTATACTTTCTTAATATGAATTTAAGATGACAATTACAACATATTCCTTGTATTTTTATAACTAGTAGCTTGCAAAGTTATTCGGGCAATTAATTTTATGATCGGAAATTCATTATGCGATAAGCAGTTTTTCGAGTTAGGATCATAAATGTTTTATCTTCGAAAAAGTATTACAACGCTTCCGGGTTTCCCAAGTTATGTGGTGCAACAATACTGAATATTCAATATTTAAGCAAAAATAAGGATAATAACTTATCTTTGGCTAGTTTTTCTCCCAGTGTCGCTGGTAACTAGCAACAAATGGGCAGTCGGCAGTTGGGGGCGTGACAGCAACCGTGAAAAGAACTCGAGGATGACAGTTATGGGTTACAAAGGCAAGGATTGAATCAAATAAATAAACGCGCAAGGTTCTCGTCATGGAGCGCGGCTCACTGGGTCGGTTATTAGGTGGCCAGGGGGACAGGGAGCCACCACCGAGTGGATTTATATGGGATTCGATTGAACGGGTTGAGCTCCGACCGGGGTGGGTTCGGGCATGGGCCTGGGGGTAACCTTACCACGTCCACATCCACGTCCATGTCAAGTTTGCATGCGGCTGCAGGTAATTCCCCATTTCACCCGCCTTTACATAATCACAACAAATGAACGAGGAAAGGCGACGGATGGCGAATCTATTAGCATAAGGGCGCATAAAACGGCGCCGGATCCGGCTGGATTCCATCGAGGATTCGCACTCGTCGACTCAGTTGCAGTGGAGCCGTGGAGCCGCGATGAACGTTTGGAAAGCAAAGGTCCTAACGGAGGTCCCATTCGGACACGTCCTCATCGTCAGTGGGCGCGTCAAGCCTCATCCGAATAAGTAATCATCTTTCATAGTTTGGATACCCGGTTCTCCACT |
TCTGGGTTAAAGTCAAAGGTCAAATGTAGAAGCTGATTTTCTAACCAAATGAAATTAACCAAATGAAATAAACCAATTAGTTCAAAAAGTAAAAAGAGATTAAGTATAAATGGTATTCGAATTATCCGATTATTGACAAAACAATGCAGTTCTAATGCCAACGAAAGGCTTATAAAGCTGTGACAGAGATTTGCAGAAATGAAATGGGTATCCTTAGCGAGTTTGGGGGAGTGCCGAGTATCCTCCCAATCCATATCTAATACTTTTTACTAATGACACCTCTCGAATTACGGACAGAATTTCGCTGGACCTCACGGACAATGTGAACGCGCAGAACGAGAGCGAAACGGTGTTCCTGAAGATCGAGGCCAATTTCCGCGAGGGCCAAATCATCCGCAGCATGTTCCAGCCGGGCGGGGGATGGCAGCAGGAGGAGATCTCGAAGAACTGGAAGTGCGACGGCCCAAAGAACCCGCTGCAGCCTGGCCAGAGCTTCACCTTCCGGGTGGCGGTGCTGCAGCGATGCTTCGAGATCTACGTGAACGACCAGCTGTACGGGTCCTTCGAGTTCGTCAAGTTCCCCAAGCAAATCAACTACGTGCGCACCTACGGCGACTTCGAGAAGATCACCCAGTTCCACCACCGCATGCTCTTTCCGCTCGTCTTCCCCAGAACGCTCATGTGTCCGGACAAGGTGGCCTTCCAGAGCGACGTGCCCAGGCGGTATGAAACCGGCACAGTGGTGGCCATGGAGTGCATCGCCAAGGGCCCGCCGACCACGGAGTTCTCCATCTGCTTCCAGTGCAACGACACCGGGCGTACGGTGCTCCGGTTCCACGTGAACTTCGATCGGA |
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