CRIMIC CR60221

Gene Or7a

CG10759

Information

Donor	Location	Cytology	FBti	Stock number
pM37_p0	X:..		FBti0219190	Order from Bellen Lab

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK

Images

Construct Design Sequences

sgRNA sequence	AGTTAGAGGATTACCAACTGAGG
sgRNA2 sequence

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right

Amplicon 5'	Amplicon 3'
GTGTGGCAACTGCTGCTGGTGCCCACATTCTTTGTGATCAGCTATCGGTACATGGGCGGCATGGAGATTACCCAGGTGCTGACCTCCGCCCAGGTGGCCATCGATGCGGTCATTCTGCCGGCCAAGATTGTGGCACTGGCGTGGAATTTGCCATTGCTGCGCAGAGCAGAGCATCATCTGGCCGCCTTGGATGCGCGGTGCAGGGAACAGGAGGAGTTCCAATTGATCCTCGATGCGGTGAGGTTTTGCAACTATCTGGTATGGTTCTACCAGATCTGCTATGCCATCTACTCCTCGTCGACATTTGTGTGCGCCTTCCTGCTGGGCCAACCGCCATATGCCCTCTATTTGCCTGGCCTCGATTGGCAGCGTTCCCAGATGCAGTTCTGCATCCAGGCCTGGATTGAGTTCCTTATCATGAACTGGACGTGCCTGCACCAAGCTAGCGATGATGTGTACGCCGTTATCTATCTGTATGTGGTCCGGATTCAAGTGCAATTGCTGGCCAGGCGGGTGGAGAAGCTGGGCACGGATGATAGTGGCCAGGTGGAGATCTATCCCGATGAGCGGCGGCAGGAGGAGCATTGCGCGGAACTGCAGCGCTGCATTGTAGATCACCAGACGATGCTGCAGCTGCTCGACTGCATTAGTCCCGTCATCTCGCGTACCATATTCGTTCAGTTCCTGATCACCGCCGCCATCATGGGCACCACCATGATCAACATTTTCATTTTCGCCAATACGAACACGAAGATCGCATCGATCATTTACCTGCTGGCGGTGACCCTGCAGACGGCTCCATGTTGCTATCAGGCCACCTCGCTGATGTTGGACAACGAGAGGCTGGCCCTGGCCATCTTCCAGTGCCAGTGGCTGGGCCAGAGTGCCCGGTTCCGTAAGATGCTGCTCTACTATCTTCATCGCGCCCAGCAGCCCATCACGCTGACCGCCATGAAGCTGTTTCCCATCAATCTGGCCACGTACTTCAGTGTAAGTCCGCTCTACTATTAGTTACCATAGTTAGAGATGATAGGTTATGAATAACAATCTTGGCTTAAGAGTTAGAGGATTACC	CTGAGGACATACTCTTTCTATTTTCCAAATACAAAGCACATACCAATTTTCTTATCCTTAGCATGATGTTTATAGATTTTCCTCTATATTAATAATATTTTCTCCATATCTTTAGATAGCCAAGTTCTCGTTTTCGCTCTACACGCTCATCAAGGGGATGAATCTCGGCGAGCGATTCAACAGGACAAATTGAAGACCATTTATCGTTGATGCACTGAGAAATACATATATATAATCTATCAAATTTCGCATAATATGCGTATCTATATATATATGTATCTATAGGATGCGCTAGTTTTAGGGATTTTAAATCCCAACTGTAGCTTGATTATCCATTCCAATATGATACACACTGTATTGTAAGCAAATAAATTAGAACACGATGTGCCAATTTGTTTTTTGTAGTGTATTCCCTGCTGTAGATATCAAGTAGATACCGAATTAAAAGCGAAATCAATAATGATCGCTAATCGGCCAGAACAGAAGCCTGCTAACCACCGAATCGCATTGGCGCACCTCTATTCCCGATCCCCAAACCCCGATCACCGCTATCGCAATACTCATCCACTTTTTTGCATCCCAAGGCGAAGCACCTGTCAGGAGCGGAGTGGCCACCTCTTCGTCGCCATCTCCATGCCAGATCCACTTGGCCACCGATTTCTGGCCCAATTCGTAGTGGTGACCACACTTTGTTGTGCTTTACCGTTGGCCCGTGCACTCAGCGAAAAATGTATATGTTATATATGTTTATATTATTGTATAGTATCAAGATCTCAAATCAGATATATAAACGTTGCTTGGAATATATGTATATAGTTATGTTATAAAATTAGTTATAAATACCTTTTTCTAGATTGAAATCAACTTTTTCTGCGTGTAGCTGTTGGCCAAAGTGGGGAAAAGTGGAATGGAAAGGCTGGCTTTCCCAGTCTGGTTCTCAAATTCTGGCCAAGCTGGAGATATTCGAGGCTGGCCAAATGTTCTTCTGCTCTCGAGCCGACAGGTTCTGCTTATTTTTCGGTCCGTCGCGAAGTTGCTTTCGTCGGAAATGTGTGTGAAAATTATGTTTTATGTTCAGTGGATTAGTGGCCAGTGCTGATCCTGCTCCTAAATTGCAGCCATAAGATAC

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