CRIMIC CR01353
CG1712
Information
PCR Verification
PCR check relative to sgRNA sequence
| HA-L |
HA-R |
Forward |
Reverse |
| OK (weak) |
OK |
|
|
Images
Construct Design Sequences
| sgRNA sequence |
CTACGGGCTTGTCCCATTCAAGG |
| sgRNA2 sequence |
|
| Amplicon PCR 5' Left |
Amplicon PCR 5' Right |
Amplicon PCR 3' Left |
Amplicon PCR 3' Right |
| CACGGTGCTGACCTTTTGTG |
CACTCGGAGTGGAGTCCTG |
GGACAAGCCCGTAGAGATTAGT |
ACACTCCGTCTAGTCTGGCT |
| Amplicon 5' |
Amplicon 3' |
| CACGGTGCTGACCTTTTGTGGCTTGATGGCACTGGTTGCATTGTTCTCTGTTGGGTGGTTTACAACTAATCAAAAATCCTGGAAATTTAAATTACCACTTACCTGCAAGAAAGCTGCTCGAAAGCATTCGGTTCAGACGGCCAAAGCGCCTTCCTAGTCCATAGGCCGTGTTGGCAATGTTAACCTGCAAGCCCGTGAGGATGAAGTAAAGACTATAGAATGGAATGTACCAGTGCACATTGAGCTCTGTCGAAGAGAAAATGAAAAGGTGGGGATTCAAAGATAGATTTCTTTCATTTTAAGATTTATTTGGCGGCTTATAAATGAATACATTAAAGCGCTAGCTACCCGTATCCGACTGAGCTATGTTCATATCCTGGGCAATGCGCATCCACGTCCCTACATCCAGGCCGACCAGAATCGAAATGGCCAGCAGAGAGACGGCAGCCATGCCCAAGGCTCGCCATCGATCTCTTCGAAAGTTATTGTAGGCATTTAGGGTATTATCGATCTGGGGTGTTTATTGGGTGATTATAACAAATAAAAGGAGGGTGGATCTATGATTGACGTGATCTCACCTTGTTTAGTCGATCGTTGAGCTCGAGAGTGTCGTTCAGGCTGAAAAGGCCACAGTAAACACCGGATACAATGGCCATGACCACCACAGACACGTCCAGGGCCGTCACCACCTTCGACGTAGCCGACTTCATTCTAAAAGATGCACTTTTGATTTGTTGGTTGGTTTTGTTTGCAGTTTGTCAGAGCAATTAATTGGTCAAGATCGAATGTGTTTAGAAGGGAGCAGAGGTGATGATTAGATAGAGAAAAATCGAGAAAAGTATACACACAATTAGGATCAAAGTAATGACACCCTCCGCAACAAAATCATATCCTTCTTGAGAGGAAAGTATTACTTATACATATTTGATAAGATATGGGTTCTAAAAGTGCAGGACTCCACTCCGAGTG |
GGACAAGCCCGTAGAGATTAGTAAAAAAAAAACGGCACAGAACTGGGACCTTACAAGTAGGAAACATAGATGTAGATACAAAAGTACCTACCGCACGGGAATTTCGGAGTTAGCATCGAACAGGAGACCCCGATAAGTGAGAAAAACTACGCAGATGCGGATAGCGTTTAGTCAGAAGGAAGTCACCCGCCTTTGGATGCTCAACTCACCCATAACCACCGTTAATGTGGCCGAGTATACAGTGAAGAGCCAGCTGCGACCAATTTCCACCCGGCCCTTGGAGTTCCGGACCGTAGCATATGGAGCCAGGGCCAGCACTTTGCTGATGTAGAAGATGCCGATGCTGGGCTGGGATATCTCCATAGTAATTCAGAACGCGGGACACTCGCAGTACGACCTCTGGACGAGAACTGTTCTCGAGTCGCCAAATACAGGACCTTAAATTGCAAAACGCCATATTCGACACCAAACAAATACTGCGCTCCCATATTTGACTATTATTAGTGGCCACCACCATCCACGCCCCACACCATGACCACATGGCCCTCGCAGACCGTCTGGGCAAACACCCCATGTGCGAGTGAGTAACACATTTGCTTATCAAAAAGCTCGGCTGTCCGCGATGTAAATAATAATTAGCGTGTGAAAATACCAAGCCGGAAAAAGGACACAGGAGCAGGAGCCAATTATTTTGGGGCCGACGTTGACAGCTGCGGCAGCTGCGACAGGTGCACCCGAGTGAATGAGGTCCACAGCCCAATTGTTACACAATTTCTCATTTATTGTTGCGACTCCACTCGGCGGTCCAGCCTAGTGGGGATCGCTATTAAGGAACAGTGGCAGATGCGCAATTAATCAAGTTAATTCTATCTGCCAGCCAGACTAGACGGAGTGT |
Search Again
