CRIMIC CR00707
CG31716
Information
PCR Verification
PCR check relative to sgRNA sequence
| HA-L |
HA-R |
Forward |
Reverse |
| OK |
OK |
AGCTACAGGTGCCAAATGTCCACAAC |
CCTCCTTTTGCTGGGCATTACCTGAT |
Images
Construct Design Sequences
| sgRNA sequence |
CAACTCGGGTATTGGCCCATTGG |
| sgRNA2 sequence |
|
| Amplicon PCR 5' Left |
Amplicon PCR 5' Right |
Amplicon PCR 3' Left |
Amplicon PCR 3' Right |
| TGAGGTGGACGATCTGACCT |
AAGCAACTCGGGTATTGGCC |
GGCCAATACCCGAGTTGCTT |
TACCTGTTGCATTCGGTCCC |
| Amplicon 5' |
Amplicon 3' |
| TGAGGTGGACGATCTGACCTTCTTTCCGTGTACCTGCGGATATCAGGTACATACTGTCATTAAAGCACCCTTATCAGCAGCGTATTCAAGTGCTATTGCTTCCAGATTTGCCGATTCTGTTGGCATAGGATCCGCACGGATGAGAACAAGCTGTGTCCGGCATGCCGAAAGGAGTATCCCGAGAATCCAGCTGACTTCAAGCCGCTGTCGCAGGAGGAAGTATGTCGTGGTCACCTCGTATTCATTCCACTAACTTCTACTCTTCTTTAGATGATTGCCTTCAAGTCCCAGAAACGCCAAAGGGACCAACAACGAAAACAAAAGATCACCGAGAACCGCAAACACTTGGCCAACGTTCGCGTTGTCCAAAAGAACTTGGTGTTCGTTGTGGGGCTACCGCCACGACTTGCTGATGCAGACGTATGTTGATTTTGCGCCATAGATAACCGACAGCAAACCCACTCACAATTGCCATTTCAGATATTAAAGAAACACGAGTATTTCGGTAAATATGGGAAAATTCATAAAGTCGTTATAAATCCAAGTACCACGTATGCCGGGGTCCAGGTAAGAGTTGTAAGATTCAATTATTTATTACCTCTATACATTCATATGGCCCAATGGGCCAATACCCGAGTTGCTT |
GGCCAATACCCGAGTTGCTTAGTCATTTTTAGGTGGTTTCTATGAAAACTTTGATAATGGCACGTTTTCAATGAAACTGAACAGATTTACGTGTATCTACCAAAATTTATTTTCGATTAAAATGAATTTGAACCTTTTGTTGTTAGCTGCTCTGGTAAGCGAACATTGTTTATAACAAGAAGTTTAAGTTTATTTTTTAACCTTTTTTATTAATATATATATGTATTTTTTTATTTTAGGCAAAATTGTGTGCGAAATGCAAAAATTTCTTGTTAATTTATGATTAAATGGATCGGAATTTACGTTCTGTTTCCGTGCTTATGCTAAAAAAATCAACTTTTGACCAATTATGATAAGAGTCTATTTTAGAATAACCCCGATTATTGTATACAAATCAAAAAGCGTAACATTTGATAAACCTGCTTTCTATAAAACTGCGAGGGTATCAGAGTTTTCGGTCCACCGAATCGCGTTTCACACTCTGCCATCATTGCTTATTTAAATTTTTTCGATTTTAAAGGGTCCATCTGCTTCTGCGTATGTCACATATGTTAATAACTCGGATGCCTTGCGGGCCATTCAAAGCGTCAATAATATTATGATAGACGGTCGGCTTATAAAGACCAGCTTGGGGACAACCAAATACTGTAGCCACTTCATGAAGAACCAGCAGTGTCCCAAGGGCGACTGCATGTACTTACATGAATTGGGCGATCCCGAGGCCAGTTTCACAAAGGAGGTAAGTTGTGAGATAACTAAATTTGTCCGTATTTAAATGTTATGTCCGGATATTTAAAGGAAATGCATCAGGGAAAGCACCTGGAGTACGAGAAGCGCCTGCACGATACTCTAATTGCGTCATTGGGACCGAATGCAACAGGTA |
Search Again
