CRIMIC CR00352
CG10119
Information
PCR Verification
PCR check relative to sgRNA sequence
| HA-L |
HA-R |
Forward |
Reverse |
| short band |
OK |
GTCAAGAAAATGTGGAATCAATAATGCACAGCAC |
TTGGCGGCGGCATTCACCGAGT |
Images
Construct Design Sequences
| sgRNA sequence |
CGAGACACAGGCAGAAAATGGGG |
| sgRNA2 sequence |
|
| Amplicon PCR 5' Left |
Amplicon PCR 5' Right |
Amplicon PCR 3' Left |
Amplicon PCR 3' Right |
| CACCCGCCTTTTAGCCAAAC |
CGCGAGACACAGGCAGAAA |
TGTCTCGCGCTCCAGTGG |
CCGCCTAAAGGTACAGTGCA |
| Amplicon 5' |
Amplicon 3' |
| CACCCGCCTTTTAGCCAAACTCTTAACTTCGTTAACGCATTGCAAAACGCACAAACAACAAATTGTGGCTATTATGAATTGAAAAAGCTTTGGGCCTCATCGCGCAGTAAATACCAAAAATAAATATGATAACGCAAACGACGCGAAACGGAAATCTCGCAAGTGGAAAAGCTAAGCAACCCAACTTTTGTTGACTTCCCACCAGAAACTGGTATTACAAATGGATCTGTAACTGAGTGCCATATGACATCATAGTCTTGTAACCTTCATTGAGATTTACGGCGGTATTTCGATTCTTCCTTTTTGATTTTCATTGGGTTTCTTTTGGTTTACTACTCACACATTAGCTGAGAACTAAACATTTGCATAAAAATGCCTAGAGAATTCTGGAAGTGTAAATGCAAAAAATAAACAACAGCGAAAGAGTCGAGAGAGTTTCCCATGTGCTTGGCTTTCAAAATGTGCTTTTGGCATGACATCATCGGCCTCGCTCGCTCTCAGTCTGCCCCGTTCTCTGTCTGTCTCTGTCACTCCCTACATATAAAAAAAAAACAACCATGGAAACTGAAGTTTGTATCTTACTAAAACTCTTTTCTTCTTACGAATAGCATGAAATATGAAAATTAAATATATTGCCTTTATAAATAAAACAATTTGTTATTGTTTTCGCTACATAACTACATAGCTGGAATTGAACTTCTAGGTCAACTGTACCTAAGAAATAAATATAAAATTCGCTTATTTCAATACAAAATACAACAAAAAAGTAAAAAAAAAAAAACAATTTGATAAAAAGTAGTGCATTTCTAGAAATCTCGACTGACTGTACTTTTACTTTACAAATCGAAGAAATTTCGCTGCTTTTCGTGCTGTGCATTATTGATTCCACATTTTCTTGACAAGGCTCCCAAAGTTGCCCCCATTTTCTGCCTGTGTCTCGCG |
TGTCTCGCGCTCCAGTGGCCCGGCATGGGTACATATGTACATATGACATCACTGGCTGATAATACGCTGTTACCAATTCCGAGCCCAAACCTGAACCCGCCGTCTTGGAGCTTTACCTGGGCTTCAAGTCGTCGTTCTCCTCCCACAGACGCTTGATATCGATCTCAAGCTTGGCCTTCTCTTTGGCGGTCTCATCGAGCAGCTTGCGAGCGGCGGCCAGCTCCTTCTCATAGACCGCCTTCAGATTAGAGGTCTCCCTGTTGACGGTATCCTGGGCGAGATTCAGTTCCTGGGTGAGCCGGCTGTTCTCGTTCTCCAGGTTGCGCATGCGATCGATGTAGCAGGCCAGGCGATCGTTCAAATGCTGCAGTTCCTCCTTCTCCTGCTGCCGGCTGGTGCGCGTGGGCGAGGTGGGTGAGGTGGCGCCCACCCTTGACGAGGTGGATGCCCCGCCCACCGGCGTGGAGGTGGAGGCGCGCGAAACGCGTGTGTTCAATGTGACGCGGCGTGCTGACATTTTTGCAATGTGTTTTCTTTTCTTTTTGCGAGGTGCGAGTTGCTAAGTTAAGTACGTAATCCTCTCAAGTCACTGTCAATATTTTTGATTCTGAATTTTTTTTGCTTGGACGTCCGCCTGCTTTGACGACTAAAAATTGACTGAAACTTTGACTCGAAACGAACGGCTATCATCGACAAAATTAGTCGTCGTCGGCGGCGGCGGTCATAAAAAACCAAATACAACACGAATGCGGCGCTGCTCTCTCTATTTTTTCCGCTCTCACCTTTTGGCTTTAGTATTATTGTTGTTTCGCCTTCCACCATATTCGCTTTCCCATTCGCATATGTAAGAGTTGTTGTGCTTTCCTATGTACAGCTGTTTGTGCCGCTGGAACTCTAAATAGAAATCGAACAACTAACAAACACACCAACTGGTCCCAAAAACGAGTTCTTTACATTTTGTTTTACTTCTCTGGAAAATCTTTAAAAATCTTGAAAATCGTGTTTTAATATAAAATGAAAGAAATTTGTAAGAAACACATGTTTTTGTTAAGTTCTAGCATTAAACTTACAGTGTTCTTTTTAAAGAAGCTGCACTGTACCTTTAGGCGG |
Search Again
