CRIMIC CR01689
CG4999
Information
PCR Verification
PCR check relative to sgRNA sequence
| HA-L |
HA-R |
Forward |
Reverse |
| short band |
OK |
TTTCGTGTGTGTGTGTGTGTGCTGTG |
TTTGAAACGGACTGTCTGTGTGTCCG |
Images
Construct Design Sequences
| sgRNA sequence |
CGTGAGGTTGATCTATATTTCGG |
| sgRNA2 sequence |
|
| Amplicon PCR 5' Left |
Amplicon PCR 5' Right |
Amplicon PCR 3' Left |
Amplicon PCR 3' Right |
| AGAAACCTCGCAACCCAGAG |
GGGTTGATTCACTACGATTTCG |
AGATCAACCTCACGTGCAGG |
GGGTCCACGCTACGTATACG |
| Amplicon 5' |
Amplicon 3' |
| AGAAACCTCGCAACCCAGAGTGCTAAATGTGGAATTTCTCTTTGTCATTGCAGTTGAAGGTAGCTCCGTGGAGGAAAACAGCCCCAAGATTTCGAAAACAACGAAAATGGTCTTCGACTGCGGAGTCTGGTGTGCAAAGTATTTGCTTTGCATATTCAATTTTATATTTTTCGTAAGTACAGATGAAGTAATGTGGGAAGGGGGTTTATTATGCTATATGCACCAGGTAATGCATATGGGTAACGCATAGAGGTGCGTTCATGTCATCCGATGGTATCCCATGCCACATCGCCCATCCATCCAAATTCCATTGCAACTCAATCAAGCAAATCTCTGCGCATGCGCTGGTGCTTTTTGCATTTTCCCGCATTTTCAACTTGGCGGTGGCGGTTCATTGACTTTCAGCCAACATCGTCATCATCATCATAATCATCATTACCATCATGTTGATTGCCAAACGTCATCATCGGCATTTTCAAATGCAATCAAGATGGGCAGGCAACGTCATAATAATAACAACAATCATCAAACTGCGTTTGCATGTCCAGCGGGGGGAACTATGTTGTGTTTAATGCTTTATGTTTCAGTGTACACACATAAATGACCACTACCACTACACACATATACATATATATTGTTTAATGTTTTTTTTTTATATATATATTTATATTGCAGGTGCTAGGCACAATTATATTCGGAGTCGGACTATGGCTGGCCGTGGACAAGCACTCGCTGATTGCTCTCCTCAAGTTGGTCGAGAGCGAGCGCATTGAGGTAAGACCAAGTGAAGACGGGGGTTTCCCCAAAATCCCGCCCAACTCCGGGTGAAAGTGTTGCCGCAGAAACACCCCCTTTTTTTATGTCGAAAAGGCCCCAAGAAAGCACTGCATTCCACCCAGTTACAACGCAATTTATACGCAACCCATCGAAGTGTGTAATTCACATATCGAAATCGTAGTGAATCAACCC |
AGATCAACCTCACGTGCAGGTGCTTCAATAATAGCTGGCACTGGTACTATGATTATTAGAAAAATATAATTATATTGTAAACATGCGGTCCGGGTGGAAATGGTCAAAATGTGAGGCACGAAAACGCTAATCAAAGTGCACAATGCACTGTGCTGACACACTAACAAACAATAGGGGATGGCTATAAATGCCACTTTAATTGGGTCGAAATTGTATATAAGACAGCAGAAATACAATGGAACTGAAAGTAGTGGAATCTAAAATTTAAATAGAGCGACAGGAAGAACTTTTCCACTACTTTTACATTTCGGTTTTTTAACTAATATCAATCTATAATTATACACATCAAGCATATTCAAAGTAAATTTTTCTTAACCTTATTTGTAATGGATGACAAAATGTATTTTTCATGGGTTAAGTGCATATAGACACATTCTGTTGTTGTGTCTACACATCTACATTTAATATAAACTACACAACATTCGACTAAACTTTTAGAAATGTACAAATCGTTGTTTAAAGCCACCCTCTGGGGTCCTTTACTCAATTCACACAGATAAACTTTTTCAACTATGACAATTTCCATCAAAGTTTGCATGTTCAGTGTTAACTAAAGTTTGTAGTGAAAATTCCCTTTGCTATCTGACTGGGCGTAAAGGGTTTTTCGCTTTGGCAAAACAAATGAAAAGTGCATGTCCATACGGTTACTAATTGTGGGGTCACCTAAGATAAACATGCTAAGGCTAATTGAGAACCTTTACACGGAAATGTTTCACGGGTTGCGTTTGGTCAATGAACTTGCACTCTAGCTTTAAAAGACATACATATCTCAGATCTGACAATTTGCCTACGGCAATTGGGTAAAATGTGCACATAATTCAATTATACGAATGAGGATGGAGACAATTTTTGGCCAGTTCAAAGTTTAATTACGTATACGTAGCGTGGACCC |
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