CRIMIC CR01707
CG8587
Information
PCR Verification
PCR check relative to sgRNA sequence
| HA-L |
HA-R |
Forward |
Reverse |
| long band |
OK |
GCTTGCTTTGTTGGCGACTGAACAAC |
ATAGGGAGCTTTCAGCTCCAAAGTGG |
Images
Construct Design Sequences
| sgRNA sequence |
CAAGTGAAACGGAGAGTGTTTGG |
| sgRNA2 sequence |
|
| Amplicon PCR 5' Left |
Amplicon PCR 5' Right |
Amplicon PCR 3' Left |
Amplicon PCR 3' Right |
| AAATTGGACACCTCTGGCGT |
GCAATTGCAGTTAGCTTGGAG |
CTCTCCGTTTCACTTGACCCA |
TCGGGGAGCTCTTGGTTTTC |
| Amplicon 5' |
Amplicon 3' |
| AAATTGGACACCTCTGGCGTACAATAATGCGAACAAACTTTTTTCAATAAGTGGACTGCCGTTCACAGTGATTTTTATGTCATAAAGAAATATATAGCATGCAATAATTTCGACACTTTGGCGTATGATAAATATCAAGAGGATAACAGGATATTTAAATGCTTTACCAAGAAAACAAATAACTTGGAACAATAAGATATGGTAATTGAGTTTAGAATGTTCAGAACATCATCTAAAGAATGTATATGAAACAATTGTTTACATTTCAACAGTTTTGAAAGCAAACCGCCAAATTGAAAAGAGCACTTTCTCTTTTAAAAACAGCCAAGAGAGCCAGCTGAAGACCTTCCGAACGTAGCTTGCTTGGATCGGCGAAGCTTGGGCCGCCAGTAGTTTGCACCAGGCAGCCGTTGAGTGTGGAACTTGAGTGCGGTTCCCGCTAGAAGCCAGTTTCGTTTCCCCGCAGATATCAGTGTGTCAACGTCAACAGAAGACAATTGGAAATCGAGGCTTACGACCAGTGAAATCTGATTTGAATTTAAATCGGTTTGGACGAGTAATGAACAATCTGTCGCTGAAAGCTTGGCGTTCCACAGTCTCCTGTGGCCCCAATCTTCGACAATGTGTGCCGAGGATCAGTGGAGCAGGTTCCAGGCGAGCGCAGTGCCGCGAAAGCTCCACGGGCGTGGCCACTTGTCCCCATCTGGCGGATTCGGAGGAGGCCAGTGCACCGCGAATACACTCCACTTCCGAGTGGCAAAACGCTCTGCCCTACAATCAAATACCCGGACCTAAGCCCATCCCCATTCTGGGCAATACATGGAGGTAACCGAAAACCAAACCAAACCAAAACCCATCATCAGAAAAACGAAAGACTCAAGACACAAACTCGCTCGCTTTCTCCAAGCTAACTGCAATTGC |
CTCTCCGTTTCACTTGACCCAATTAACTTGGCCAGCTTGTTCAACACTTATTGAATTAGCCATGATGATGTTGTTCGTTTGTTTACTTGGATTTTGTGCGAAAAACACGCAACGTCGTCTAGTTTTTAGCCCACCTCGTAAAAATGATGTATGCTAACTAAATATTTATGTGGGACTTTGGTTTCCCTAACGATTCTTGTTAAATGTGCGTGTGGGATTCCATCAATTTACACCTGTCCAATTCCAAACCTTAAGTTACAATCCGTTCGATGGCACGTCATCCAGTTAATTAGCGTTCAATTAGCGCTAAATTCAAGGTCGCCCTAATAACGTGTGAAATTTTGCTGCATGCTGACGAATTTTGCTCACAAATCATATTTTTTCGCCATTGGAAAAAGTCAATGCATCTTTCATTGTCGTAGATAGTTTGTTTAACCTTGACTATTCCATAGACTGATGCCAATAATTGGTCAATACACCATCTCTGATGTGGCCAAAATATCATCGCTGCTGCACGATCGTTATGGTAGAATTGTGCGTTTTGGAGGTCTTATAGGACGGCCCGATCTTTTGTTCATCTACGATGCCGATGAAATTGAAAAGGTAAGCTTTAAGACACGCCAGCGGCTTGGGACTTTCCGCATGACCAACGAACCACGAAGGCACCAAGCCATTACACAAATTTATGGCACCGTACAAATTGCCCACAGTTGTCGGATATTAAGCTATAGCAATTTTATGAATTTCCAGTGCTATCGTAGCGAGGGACCCACCCCGTTCCGTCCCTCGATGCCCAGTTTGGTCAAGTACAAGAGTGTGGTGCGCAAGGATTTCTTTGGGGATCTGGGCGGCGTAGTCGGAGTGTAGGTGGAATTGCCTCATTTGTTGAGCTTTTAATTTATTCTGCCTTAATTCAGACATGGAGAGCCTTGGCGGGAGTTCCGTTCGCGTGTCCAGAAACCGGTGCTGCAGTTGTCCACCATTCGCCGGTATCTACAGCCGCTGGAGGTTATCACCGAGGACTTCCTGGTGCGCTGCGAAAACCTGTTGGATGAAAACCAAGAGCTCCCCGA |
Search Again
