CRIMIC CR00719
CG6510
Information
PCR Verification
PCR check relative to sgRNA sequence
| HA-L |
HA-R |
Forward |
Reverse |
| OK |
no band |
GATTTCACGGAAGTTCGCTGCACAAC |
GCGGCAAGTCCATCTATGGCAAAAAG |
Images
Construct Design Sequences
| sgRNA sequence |
ACAAACACACGCATGGCTGTTGG |
| sgRNA2 sequence |
|
| Amplicon PCR 5' Left |
Amplicon PCR 5' Right |
Amplicon PCR 3' Left |
Amplicon PCR 3' Right |
| ATCGTGGAACTGCTTCACGT |
TGTGTTTGTTTGCCTGCGAC |
GTTATGTTTTCGGCGGCACA |
TAGTTCGATAGCTGGCGCAG |
| Amplicon 5' |
Amplicon 3' |
| ATCGTGGAACTGCTTCACGTGCACGCGGCGGGTCTTGGCGGCAGGGATCGAGTCCACCTTAATGATCTGGATGGAGTGGGCACGGGCACGGTGGCGGGCTCCCATGTCGCGGTAGCACTGAGTGACGGCACCGCCGACAGTCAGGTCACGGTACTCGCGGTACATGTTGTGGGTGCCCGAGCGGGAATCGTAACGCAGCCAGATGCCGAAGTTCTTGATCTTCACGGGCGACGTCTCGTACACCTGCTTGATGGACACGATCTCGCCGGTGGTCTTCTTGAACTTCTTCAGTTGGCGCAGGAAGTACCAGAAGCGGGATTTGGCCACGATGTTGTCGGGGGCAAAGATGCGCATCTTGTAGAGGGGAGTCTGGGGCTCCTTCTCGCTGGGCAGCTTGCGGCCCACGACCTCGTACTCCTTCAACTGCAAATGGGATTTGGATTAGTTTAAATGGTCTACATAAAACAATTGTAAACAAATGCGAACAGTCGCAGGCAAACAAACACA |
GTTATGTTTTCGGCGGCACAAAGCAGTTGTATTTCTATTATATTGAATATGTTTGAATAGTTAGTTATAAATTCGTACTGAATAATAGTTTCTCACAATTATGTAAATTACTTTTGTGAGTTAGATAGGATCACATTTTTAAGAAAAGTGGCTCGGCAAACATTGTCCACGACAATGTCGTTTTTTACACAGAAATTACGGAGTTTTAAAAAATCCAACGATTACGCCAAATAGGCTATGTTTTTGCTTATCTGTTGCACGCATTTTAAACTAAAATAATGATTGTATAAATCATTTATTTTGGCAAATACTAACCAATCCCTTGGCTCTCATGTTGGACGTTCACGCGAAAAGGAAACGGAAAGAGCTTGTCAAATCGGTATGACACTGAACCTCGATTCAGGGATGGAAAAAACTATCGATAGCTAACTACGAATATTAGTTATGATTGATCCACTGTGATACTGGACGTGCTATAATGGTTCGTTTAAAATTAAGATAATTACTCAAGATAATATTTTTAAATAGCCCGTGTAAATAAGATTTTAACAATAATTGCTGGTACTGGCCAAGTATCGATATGCTTACATTGAACCGCAAGGGAAAAGCACAGCGATGCCTGTCACACGGTTGCATTTTTGGCAGTCACACCAACACGCTGCGCCAGCTATCGAACTA |
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