CRIMIC CR00508
CG4400
Information
PCR Verification
PCR check relative to sgRNA sequence
| HA-L |
HA-R |
Forward |
Reverse |
| short band |
short band |
TTGGCGATTCGCTCGAGCACCATATA |
CCAAAAGCTAAGCCAGGTGAGCAGAT |
Images
Construct Design Sequences
| sgRNA sequence |
CCATATCGTCGCAATTAGATTGG |
| sgRNA2 sequence |
|
| Amplicon PCR 5' Left |
Amplicon PCR 5' Right |
Amplicon PCR 3' Left |
Amplicon PCR 3' Right |
| CTGCGCCACGAGAATCAATG |
TGGCTTTCGTGTTGATAAGGC |
ATTGCGACGATATGGTGGCC |
CAGCTAGAGATGGCAGACGG |
| Amplicon 5' |
Amplicon 3' |
| CTGCGCCACGAGAATCAATGAGCTGAGCGGTCAGATACACTTCCATGGTGACCACGTGGACGTCCTGCGTGACTATCTCAAGGAGAAGGGCTTCTGAAGGTCCACAAATGAACCGTCTGTGCCGCAATAAAAGCTTTTCTGTTGATTTAAATCATGTGGGATTTGTGTTCTTCTGTCTCGCCTCACCTAAGGCGGCTTACCCACATTGTGACCTCCTACCCATAGTATCCAAGTCTGGATAACAAAACTGCTGGCCTTCCGGAGATTGAATCACTCGCAGGCAATCGAATTAAATTTATGTTGAACGCATGCCCACTTGGCTGCAGTTCGCTTTTATTTGCACTTATCTTTTAGCATAAATTACATACATCGTCTGTATGGGGGAGGGGAGAGACACACACACACACACACACACACAATATTCTGAGAGTGGGACTCGTACAAACTACGTACGTACTAAAATCTACGGGAAAAGGAGGTGAGGATGGGTGGCGGAGCTTAGCCATTTTGATGAACGCATTTGAAGTCTGGTTCGGGGGCTCTGCTACGGCTACTACTGATACATAAACGTCTACGGCTGCTGCTTCCTCGGGCGCGGCCGTCAGGTACGCGTCATGTGGGCGTAGGCGGCAGCTGCCACGCCCCAACGCTATCCGCTAGCTCCTGCCATTGCTGGCAAACCGGACAGGCTAACGCTTACGCCGGAAGTGGGCGTAACTGTGCCAGCAGCGGTGGCCGACGAGGAGGATCGCTTGAGGGCCTTGCGTATAATCGTCCAGTCCTCCATGATATCCTCCTCGCGCAGCATGTACACGACATAGGGACCCGTGACGGTGACGGCCTTCTTGCGACCCGGTCCGCGCACCTTGCTCTGCCGCTTGTCGGTGCCCCAGTCGGCCCACGAGATGTCCACGTTGTGGCGATCCTCCTCCAGGCGACGGATGCGCTCCATGAACTCCTCGCGCAGATTGTCCAATGCCATGTGTTTCTCGCTCTGCAACGGAAAAGTTGTGAGTTGCAAGAGGATACATTAACAACTACTTCTACGCTTAAGTTAAGCCATCATTTGTCTTAAATCGTTAATATATGGCCTATAAGTGAGTGACATTTTGTGTTGTAATTAGCTTTGGCAGTGTCGCCCCCAGTGCCTTATCAACACGAAAGCCA |
ATTGCGACGATATGGTGGCCAATTAGCTCTTCTCATTTTTGGCGCCAAAAATCGAGATTGGTTATGTACCTCAAAGTGCTGGACGGCAGCTTGTTCCTCGGACTGGTATTTGTGCTCGATATTCTGCAGTCGGTACTTGCGCAGGACGTCCGCCACCTCGATCCTGGTGCGATACTTCTTGTCCAGTTCCTTTTGCGGCTGGACAAACTCCTCGGAACGCCCGGAACGCACCTCGGCCAATTGTCGTTCGATGAGATTGATGCGCTCGACGTAGTACTGTTCCCGCAACTCGTTAAATTGGCGCTCCAAGCTCACTGTGTCAAGGAAAGAAGAAACTCGATGAGTATGGCTTGTGTTAAGTGTGTACGTACTGATTCCTACGCAAATCCTCGATGTGCTCTGCCCGCCGGCGGTCTATTTCACTGGCATCCAACTCGGAGGAGTCATCCGAATCGCATTCGTTGGCCTCCTCCTCGTCGGAGGTGTCGTGTGGCTGGCTGGAATTCGAGTGCTCCGATTCTCCGCCAGAAACGTCGCCTTCGCCATCGGATTCGCCATTCTTCACGGGCATTTCAGAGTGGATTTTGCGGTTCTCCTTGTTCTCGGCGTATTCCGATTCGGCAATGGACTGCAGGTGGCCGCCGGTATAAGCGCGATATCTTTTTTTGTTTGCTTTCGACAATCGGGGAGTGTGGAAACTGTGGAAAACTCTGTGGAAAAACACTAAACTGTGTGCTGTATCAAAAAAAGTATGACCGCACGTAAGAGTGTTGGGCAATTTAGTGTTGGTCAACGCAAGCGGAGCATCTAGCTGAGTGGCTACAAAATAGCTAAATCTGCGAAAAATATTATTCATTAAGTTAAACACATTAAAATATTAATGTATCATTGAATTTGAAGTAACCTGGACCACTAAAATAAGAGAAAATAAATTTGGAATTTACCCATGTAACTATTTAAATATAAAAATGTTACAATATTTTTGTTTGATGAAAAATAATGATATTAACTCTGTCGTAAATTAAATTATAAATAGAAGGTCATGGAAGTAATTTCCGATATTTAGATTAAAGGAATACATCTTTAAAATATGGTTCATTCCCATATAATATTGTGGACTTATTTGAAATAGCCAAATCTAGGCATTTTCGGCTGCTCAGCCCCACCGCCGTCTGCCATCTCTAGCTG |
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