CRIMIC CR01143
CG5853
Information
PCR Verification
PCR check relative to sgRNA sequence
| HA-L |
HA-R |
Forward |
Reverse |
| OK |
short band |
GTCTATAACAGCACCACAGTGCCAGT |
AACACCGCACTGGGATTATGGAACCA |
Images
Construct Design Sequences
| sgRNA sequence |
ATCATCTGCAGGCTATAACTTGG |
| sgRNA2 sequence |
|
| Amplicon PCR 5' Left |
Amplicon PCR 5' Right |
Amplicon PCR 3' Left |
Amplicon PCR 3' Right |
| TGCACGAGCTACTGTCCAAG |
TCATGAATATCGTCGCTCGTAC |
ACTTGGCTGAATTAAACGGCT |
TCCCTCACCGTCAAATTGGG |
| Amplicon 5' |
Amplicon 3' |
| TGCACGAGCTACTGTCCAAGCCACTGAAAGAAAGAAAAGCAAGATGTAAACATTCCTCAGTTTGTTAAAAATGGATATATTGGCTCATACGTTGTGGGCTCGTCCAGGAACATCACAGTGGGATTATTGATCAACTCCATGGCAATGGATAGCCTCTTCTTCTGACCACCGGACAGGCGCATTGTAAGGGTCTGATCGTGATTGTACAGACCAAGGAGCAGCAGAATGTCCTCGATCTGCAAGGGTAAATCCGCGATTTATGACTATGATCTTTAAGTGAAGATCACCCCACTCACCCTGCTCTCCTTCTCCTCGTAGCTGACTGTCTGACCCAGTTTAAGATCGGCGGCTATATGCATATTCTCGTTTACCGTCAGCAGCGGCTGCAGACGATCATCCTGGGTGATGTAGCAGGACATCCGACGGAAGGAAGCTGCAGGGAGGGATTACCATGAATTCTGGATATATTGAATAAATTAAGTGAGGTCATATACTAACGCAAATCACGTCGCCGACCATTGAGCAAGATGGAGCCATCCACTCCGGTCGTCTTGAAACCTGAAAGGGCATCTAGCAAAGTGGACTTTCCTGCACCCGAAGGTCCCATGATGGCAATCAGCTGGCTGCCTGGGAACTTTCCGCACACATTGTGCAAGATTTCCTTGGATCCTAAGAAATTGCGAAATTACTTAAATGCCATCGTAAAATATGATCATAAAAACTTAATAGACATTCAGTGTGTTTTTTTTTTATACGGTCGTCGGTATCAAGGTTCAGGGAACCAATGGAGAACCCATGAGTACGCGTACGAGCGACGATATTCATGA |
ACTTGGCTGAATTAAACGGCTATTGAGGTAAGATCTCGAGTAAATCTACATTAATCCACTTCAGTGAATAACCTGTTAACTACACAACTCGAAAACAATTCAAATCAGTAAACAATTCTAGGTGATATACGTATCAAGCCATAGAAAAGAGTTGCAAATATAGGGAAAAAGGGTTTAGCTTAATTAACTCAATCTTTCACAGTAATCGTATTGTTATGTTAGCAATATTACACATTTTAGCACTTCTAGTCACCAATTTAACATTCCGATAGCAATGGCCATATATCCACCTACCGAAAGGTTGATTAATTAATTACCGCCACGGGTTGAAAATTTGTGCAGTTCTAATCAAGTTACCAGGGGCTAAACAATGTCGATATCTTTTGAACTTTGAAATTAGCAAAATGTTCTAAAGCCAAACCCATCATATCGAATTACAAATGCTCTACTAACTATCGTATTGGCAAGTTATTTCTTATCATTTTATGGACTCGACGGAAAGGGAATTTATGAATGACTAACTGGCATTGAATATATACAACCGGGAAGGGGGTGTGATCGGAATAAGCGGAGTGATCGGCTCTTATCTAATCAATGGAAAACCAAGTTGGTTAAAGAACTAGCAAGCAAGTGTTTGGCAAGTGATTGGTATGTGGAGGCACTGCCGAAAAGAATAACCTTGTAGTAAACTTTGTTCGGATGTAATTATCTTATGGCACTCGTACTTAAGAAACATTTGAAATATAGTATCTGAAGGATTTGCTTTATCTGAGTATATCTGTATCCCTTAGAATACTATTAATGAAAAGACTTTCTAGCATATGTTTTATCTTCTATTATAACAGTAGTTTTGTCTAGTCATAATTTAGATATTATTTATGAGACTATATTGGTAGGATATAAAGAATTGAAGGTCATTCCCGTTCAAAGAGTAGCCGCTGATCGTAAAACATATATAAATGCCCAGCTAATTTTAAGTTCTCCGCTGCCCCAATCTTATCGAGCCATATCTTAGATCATGCGATCGCAATGGTTAGTTAGCGACAACTCGCTTCTTGTAGCTCACCTCGATTGAAGCCCAATTTGACGGTGAGGGA |
Search Again
