| Donor | Location | Cytology | FBti | Stock number |
|---|---|---|---|---|
| pM37-p1 | X:15155975..15155995 | 13B1 | Order from Bellen Lab |
PCR check relative to sgRNA sequence
| HA-L | HA-R | Forward | Reverse |
|---|---|---|---|
| OK | OK | CCAAGCTTTGATTTGTGGCTGCCCAT | GAAAGTGCAAGTGCTAGTACCCGATG |
| sgRNA sequence | TCTTCAGAGGGGGTGTCTTCGGG |
| sgRNA2 sequence |
| Amplicon PCR 5' Left | Amplicon PCR 5' Right | Amplicon PCR 3' Left | Amplicon PCR 3' Right |
|---|---|---|---|
| TCTCACGCAGCTTCTTCTCC | GGGTGAAAGATGCTGCCTTG | ATCGCTAGATCGGTGGGTCT | CTCCGGCTTTGTGTTCTCCT |
| Amplicon 5' | Amplicon 3' |
|---|---|
| TCTCACGCAGCTTCTTCTCCTTTGTGGCGCGCTTCTCTTGACCCAGTTTGCTAAGCTTTTTGGTGTTGCTGTTTGCTGTTATTTTTTTTTTTGTTTTTTTTTTTGGTAAGCCAAATTAGTAGAACCTCGTTAAAATCGCACACAAACAAACCGACACACACTCGGAGAGTTGGAGAACATCGAAAAAAGATTTCGCAAGTGAGTCGACTTGCGCAATGTGGCCATTGTTGCTTTTGTTGGCGGATGCGCCTGGCCAGACGAGACTTGTGCAAGCTGCAAGTTCGTTGCCTAAGTCTTTTGATTTGAACTTCCGCCAGAGGATCTCACTTGGGCGACTTTTGGGCACTTATTGCTTGATGAGCACTAAGCGAAAATTTTGTGCCATATATATCATCTATAAAGTTAATTATAAAGTCACCTCTTGACACTTACAGGAGAAAAGCAATTATAGAGAGCAACGTAAATTTGAAGTATAGCTTATAGCATGAAGTATAGCATAATTCTAAGATCGCATTTGGTATTTCAGTAAATGACAAAATGTATGATGATAACACATATAATTAGGTTAGTATAAAGTCAAAGTCCCTAAGCTATTATTAAACCCAAACATATTTAATTAAGCAGGCCATGGTTTTTTTTTTCGGTGTCTCTGTCCGGGGGACTGCCCTTGGCTTTTGGTATTCCTCGCCAGCCGTTCGTTCTTGCCTTTTGGAATTAATTGCGCATAAAAATTGCCCATCATGCGCAGTAAATGAGACATCAAAGAACGTCTGCGAGCTAGTTGGCTGGCTAAGAGCTGCTAAGCGTATTAAATAAACATGCCAGCGATGGTTTCCCCCCCCCCCCCCCACCACTGCCACCGCTCTTTTATGTAACGGTACGCACAGCAACAATAAATTTCAAGCGTAAACAGTTACGTAAAACAACAAGAAGAACAACAGCAACGGCGGCAGTGGTTGAACAACCAGAAGAATTGCTGCTGGCTGTCTTCTGCTCACCACAATGACAGCATCCATTCCATACCATTCCGTTGGCAAGGCAGCATCTTTCACCC | ATCGCTAGATCGGTGGGTCTAACTCGTAGCTTTATAACAGGTTCGATATGATTTTGCGGCACTTTTGCCGCAAAGGAAACGCTTGATTGCCGCTTCTAAACCAAGGCCAAGCAACGGCAATCGTCAGCGGACCATCGGCGCAAGCCAAAAAGACGATCGACGACATTGACCGACGATGCTGAATGCTGAACACTGGACTCTAGACTCTAGACTCTAGACTATAGACTCTAGACCATAGATACTGGATACTGGATGCTAGGTGGTGGTAGCTGGATGTTGGAAGCTGTAAGCCGTCTTTGCCGTTTGCCAGTTGACCGCATTCCAAGCACCAGCCAGCAAATCAATGTGCAAGCGTAAAAGCAACATCATCAGCCCAGTTGCGGTTGTCCTTCTCCTCGCTGCACAAAACAAAATCATCGTATTGTACTTAACATTATATGTGTATAATATTATACAACTGAAAGGCAAATTTAACAAAAAATTATTAGTTACAGCTAGTGCAACTAAATATCAAAATATTCTCAAATTTTTTCTATAAAAAAGAGAGCAAAAGCATGCCCAATTTGTTTCACTGTGCGCTGGGTTGCAAACTGGGTCACTTCGGCCCAAATGCAGTTTAAGATAGAATGCCAGCTGGGTGTGTGTGTGTGTGTGTTTGCCAGCTGCGAAACACCTGCAGTTGCAGCAGCAGCAGAAAAAGCAACTGCAGCAGCAACATCGCCGCTTTTCAGCTCGTGCCACAGTCAAGACTTCACAGTGAGTGATTGCAGTGGGGAAATGTGGTGAGCGGTGGCACTTAGCCGGCGCCCACTGTGCACTTTAGATGCGTTCTCATTGCAGTTGCTAATTTCTGTGGCTTTTGCATCGGCTTTTGGTTTTGTGCCTAATTTGCTTTGGCTGTGAACGTTCTCAATGCTATGCGAAGATTGCCCCCACTTTGGTTTTATGGGCTTTTTTTTTTGGGTTTTTGTTTTGGGCTTGTGGCCTGTGTCTGCCACCAAAAGTGGACAAACGCTGGGGTTAATCTTTTGGTCAGACCGCTTCCAAAAACACACCACACACACACACACCAGTGCAGGCAAGTAGTTAAAAAGAAATGGAAATGGTAATAGAAATAGAATAAAAAACAACAGTTTACAGGGCAATTTGCATAAGAAAGGAGAACACAAAGCCGGAG |