CRIMIC CR01610
CG8404
Information
PCR Verification
PCR check relative to sgRNA sequence
| HA-L |
HA-R |
Forward |
Reverse |
| OK |
long band |
AGGCAGTCAACACCCTGTAAAACGTC |
ATTGTGATTCATGTGACGCTCTGCCG |
Images
Construct Design Sequences
| sgRNA sequence |
CCTATGCCCCAACCTCCAACTGG |
| sgRNA2 sequence |
|
| Amplicon PCR 5' Left |
Amplicon PCR 5' Right |
Amplicon PCR 3' Left |
Amplicon PCR 3' Right |
| GGGGATACTCTGAGTTGCGG |
AAGTCGTGCCAAGTCGTTTG |
AGGTTGGGGCATAGGTAGGG |
CAGCACTGCCTCCATTTCCA |
| Amplicon 5' |
Amplicon 3' |
| GGGGATACTCTGAGTTGCGGACAGGTATGTGACAACAATTGGGCAGATTTGTCTCGGATTCAGAACAAAACAGAGTGAAGAACAATGGCATTCTATTATTCGGGAGATTTTGTTGCTGGGTATGTCATAGAACGATTTAGACACTGATATGGAAATCAGTGCATATTTCGAAATTATCGTGTAACTGGCTTATCTGAAAATTATGGAATTATAATAGTGAGTTCTAATTACTGCGCTTTCTAATCTGATCTAGTTATCCATAAGATTAACATGTTGGAAATGGTTATTTTCCTATTAAATTTAATTATATACCAATTTCAATTATGCATGATTAGGCTCTAGTGCCAGCGTTCCAATTGAGATAAATCCCATTGGTAATCCGATTAGCTTCATATACATTTTAGAAAGCTTCTGCCCAGCAAAATGGTAATGAACAACCTTGATATATAATTTATGTAGATCTGATTGCGAGCTCCGCTTAGAATCCAATCTAATGCTCGATTGCATAGTTAGGCATCCGAGATTTGCCCACCAAAGAGATGCATGCCTAGAATGGCAAATCATTTGGCGCCTCAACTGCGATCTCGTATTGGGCCAGTTTTGCAGCCTGGCAGCCTGTGGAGAAATGCAAGCGGTGCGGCAAGGTAATAAAAGCCATTTTGGTTTTCGGCCACAGTCAAAGCCGGCTCGGCTCGACTGGCGAGGCTGAATCTGGATAAAGTGGCGGAGTAGGAGGTGGCTGGTGGGACCTGGAGGTGGGTGTGGCCACAGCGCCTGGCGAAGAGGCATCTGCCGTGGCTCTCCATTTGCTCCATTTGCAGTCTATTGGCTGCCTGCTGCGGCTGCAGGCGCCCCGCATACAAAACAGATTTCCAGAGGCCCAGCACAGCACAGTGCACAGTGGAGCTCAGCGCAGGCCAAGAAAACGAGTTTGGCCCAAACGACTTGGCACGACTT |
AGGTTGGGGCATAGGTAGGGGCGCTGGTGTATGCCAGCATGGATAAACTTGTGGAGCTGGAGCTGCGGCCCAGGTGTGATGTACTTGAATAACTTAACGACGCAGGCCCGGTCTTTCAGCGGGCCGCGATGAGTTGTCGGTCGGTCGGTCGGTCGATCGGATCGGCCATTTACGCATACATATGCATGGCCGCCTATATAATCGTACGTATATGCATATATCTGTATGGCCGCATGTAGATGCTGTTGACCAATAAGCATAAACATCCCAGCATCCGGGCCTAAAGAGCCGCCATCTTAGCTTTAATGAACATGAACATGAAGGTGAGCTACCTGCCTCGCTGCGAATGGGAATGAGATCGGTACGCACAATGGATTGGGCTATAAGGTATAGGTACCTCCATACCTGTGCCTTCTATATAGAGGCGATATATGTGTGTATGTATGTGTGACCTGGCCTGGGCATACCTAATTAAACTAATGAAGCAAGCGAAGAGAGTAGCGTCATCATCATCATCACCATCCATCTTCGAGGGCCGAACGTTGCCGAAGCTGCTGCTTTCGTTCGGCTAATGAGGTTTATTGCGGCTGTCGTCGCTGTGGATCCCCCCAGCCCCACTTCTTGAGGCACCACCCACCCCCATGCCATGCCGAAGAAACAACGAAGACCCAGTTCTCACACAAGCGATTTTAGGAGCATAAAAAAGATACAAAAATTTAATGGATTTTAGCACGCACACCAGCACACGCAGCCAACAGATACATGTACGTGCTCAGATACAGATACACATACAGATACTTATACGTATACACCCTCGCAGGTGGAGGGGTACTAAATTATATGGATTGCATGGAAATGGAGGCAGTGCTG |
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