CRIMIC CR01878

Gene Hr96

CG11783

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p0	3R:25025235..25029351	96B16-96B17		Order from Bellen Lab

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
short band	OK	GAAGATAGTCGACCAGATCGTATCCG	CACGTCTTTGTGTATCACTCGCGATC

Images

Construct Design Sequences

sgRNA sequence	TATCTTCGTCAGCCAGTTTCTGG
sgRNA2 sequence

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
AACTTGATGCACTCGCCAGA	CTGGCTGACGAAGATACGGC	AGCAATGCTCGCCTACCAAA	TCAGTGCGTATGTTGCCCAT

Amplicon 5'	Amplicon 3'
AACTTGATGCACTCGCCAGAGGACGCCATCGATATAATGAACAAGTTCATGAATACCCCAGCGGAGGCGCTGCGCATTCTTAACCGAATCCTAAGCGGCGGAGGAGCGAACGCAGCCCAGCAGACAGCAGACCGCAAGCCATTGCTGGACAAGGAGCCGGCGGTGAAGCCTGCAGCGCCAGCGGAGCGAGCTGATACTGTCATTCAAAGCATGCTGGGCAACAGTCCGCCAATTTCGCCACATGATGCTGCCGTGGATCTGCAGTACCACTCGCCCGGTGTCGGGGAGCAGCCCAGTACATCGAGTAGCCACCCCTTGCCTTACATAGCCAACTCGCCGGACTTCGATCTGAAGACCTTCATGCAGACCAACTACAACGACGAGCCCAGTCTGGACAGTGATTTTAGCATTAACTCAATCGAATCGGTGCTATCCGAGGTGATCCGCATTGAGTACCAGGCCTTCAATAGCATACAACAAGCGGCATCGCGCGTAAAGGAGGAGATGTCCTACGGCACTCAGTCTACGTACGGTGGATGCAATTCGGCTGCAAACAATAGCCAGCCGCACCTGCAGCAACCCATCTGCGCCCCATCCACCCAGCAGTTGGATCGCGAGCTAAACGAGGCGGAGCAAATGAAGCTGCGGGAGCTGCGACTGGCCAGCGAGGCTCTTTATGATCCCGTGGACGAGGACCTCAGCGCCCTGATGATGGGCGATGATCGCATTAAGGTAACCAACAGTCCACGGTATTAGCCTATAGGTCTTTCTACATTTATAGCTCCAACACCACGGCTTATCTAATCAGAGTGTGCGAGCTGCGATATATGTACACACGGCACCTGGCACTTTTTAGCCATTCGGTGATTCAGTGCGTCTCTCGATGTTGGCCCACGGGCCGTATCTTCGTCAGCCAG	AGCAATGCTCGCCTACCAAATGTAAACACACTTTTTAATGGGGTGGCTCAAAGTTTTTGATTTCCCAAGAGCTTTGGTCGAGTAAAAGAAAATTGATCGAACCAGATAAGCTATTTTCCCCCAGAGGGTTAAAGAATTTGAAGTCATGCGACTGGGTCTAGTTAAGATATTTGATTACGAAAATTGGCCTTTAATTAAGACCCTAAACGTGACAAACTTCCATTCTATATACTTCTTGATGAGTATTTAAACAAATATGGCTATTTTCGGAACAAATCGGGCACTCATTTATATCTTTAGCTTTATCTTTATTTTTTAAGATGTGTCCACACCTTTGATCGACCTCTAGTTCCCCTGGAGAAATGATTTGGAATTATCCAATAATGATTCATCACTTCCACGAATTGTTGTCCCATTAATCGAGCCACCCTAGCTTTCATGCAATCAGAACGTCTGGTCTGCCAAGAAGGAGCAGACAGCGGCTTTATCAGCCTCTGGGCGTGCCAATTGTGACACTATCAACCATTATCAAGAGTACCAGCAGGCGCTCATGAGTCTCCAGCCAGCCGTCGATTTGGGCGTTTATTGTCGCTTAGACTGTTTACCGATTTTGCCTCATCGCAATTAGCACATTTCAGTATTGTTAATTGGGAAAAACGATACAATTTTGACGAAATATATGGAGCAGCCAGGTGTTGGGCGCTATGATAAGCAGTGCTCCGCCATTCGATTGAGTCACCTTCCAGGGAGAAGCCTTTACGATTATGGCGATAATAATGGCCACCTGTTCGTTCTTTTCCATTTTGTTTGTTTTGTGAACCACAGCCCGACGACACTCGCCACAACCCAAAGCTATTGCAGCTGATCAATCTGACGGCGGTGGCCATCAAGCGGCTTATCAAAATGGCCAAGAAGATTACAGCATTCCGTGACATGTGCCAGGAGGACCAGGTGGCCCTACTCAAAGGTGGCTGCACAGAAATGATGATAATGCGCTCCGTAATGATTTACGACGACGATCGCGCCGCCTGGAAGGTACGTAGCCTTAATTAAATAACTAAATATTCTATTAGACTGATATGATATGATACAATGTGCTCCTACAGGTACCCCATACCAAAGAGAACATGGGCAACATACGCACTGA

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