| Donor | Location | Cytology | FBti | Stock number |
|---|---|---|---|---|
| gRNA_int200_pM37_p0 | 2R:17001994..17003599 | 53E4-53E4 | Order from Bellen Lab |
PCR check relative to sgRNA sequence
| HA-L | HA-R | Forward | Reverse |
|---|---|---|---|
| OK | OK | TTGTTAGTGTGACCGTCATGCG | TAGTTCGTCGCTTTGGTGAGGATC |
| sgRNA sequence | ACAATTCGCACGTTTGAAAAGGG |
| sgRNA2 sequence | GATCACATCTTGCAGCTGTGTGG |
| Amplicon PCR 5' Left | Amplicon PCR 5' Right | Amplicon PCR 3' Left | Amplicon PCR 3' Right |
|---|---|---|---|
| Amplicon 5' | Amplicon 3' |
|---|---|