CRIMIC CR01831
CG8727
Information
PCR Verification
PCR check relative to sgRNA sequence
| HA-L |
HA-R |
Forward |
Reverse |
| OK |
OK |
ACCTGCTCCCATGCCATCATGATCTA |
TGTGGAAGTACTCGTAGAAGCTGGTG |
Images
Construct Design Sequences
| sgRNA sequence |
AGTGTGGATTTCCCGTGTAACGG |
| sgRNA2 sequence |
|
| Amplicon PCR 5' Left |
Amplicon PCR 5' Right |
Amplicon PCR 3' Left |
Amplicon PCR 3' Right |
| GGGTACTGTGGAGTTGCGAA |
CACGGGAAATCCACACTGG |
CTTCCTCTTCCCTTCACACCC |
AGTTTCTCTTGGACCTGCGG |
| Amplicon 5' |
Amplicon 3' |
| GGGTACTGTGGAGTTGCGAACGTTGGGCGGAATGCGACCAATTGCCACAAGGCAGGATAGATTCGTTGTTTGCTCGTCGCTGTCGGCGTCCTGGTCCTCGTCCTTGATGGGTGTCCAGGACTTGAGGTAGCCCGTGCACTGGATAACCCGGTACTTGTGGCCCGTAGTCAGTCTGGACTTGCGCTTCGTGGAGCTCCGGGAGCTGGAGGACGTATCGGACTCCTCCTTGATCTGGTTGTTGCTGGCAGTGCGCAGCTTCATGCGGCAGAAGAAGGAGCGCCGGGCACCCGGACACAGGCGGCACAAGCTCTGTGGAACGTCGGTCTTAACGGGCAACATGGCTGCAGGTGATAACGCAAGGAGAAAACTTAAGTGACGTACTGAATCTCACACTTTCGCATGGCCACTTACTCTTCGCATCGATAAGCCTTTCCCTGGGACACTGTTCCAGTGAGGATAGCTGCTCCTTAACCTTGCCTATGTCCTTCGGATGCAGGACGTCGAACCAGCTCTGTCCCAGCAGGTCCGCTTGGGTGCTGTTCAGCACACTGGACACCGAATCGGAAACGTACAGGATGCGTCCTCGGTCACAACCCACCACGAACAGGAATCCCTCCGACGCTTGCAGGATAATCATCTTGAGCTCCTGGTCGGACAGGAAGCTAGGCCGGTAATCGGATCCGTTGAATGGATGTAAGCTGCCGCTGCCACGGATCCCTCGCAGATGCTGCACTGCCATCCGGAGCACAGTCAGTTTGTCCAGCTTTCGCTGCATCGCAAAGCACATGGGAATCATGGAGGAGAGCTCGTTGATGTACGTGTTCATCTTGTCCCGACGCCGCTTCTCGATCTCGCTGTGATTTTGCCTAATTAAAATAAATGATATAACGTTAAAATTATAAATGCATATGGATGCAGGAATCTGATTGAAAAGACCAATTAACTTTTCCGAAATAATCATTATTAGTCTTGAAGTCGAATAAATGGATATGGAATTAGTTAAGCCTGAATAATGTTTTCCGTACTTGCGATTTTCATCCGAGGTTCTAGCTGACTTCTCCTCGTCGTAGTTTTCATCTTCGATCTCCTCCATATTTTCGCTAAATCGAAAACAACAAATGGTGAATTAGAAAACAACCCCGATATTTAATCAACCAGTGTGGATTTCCCGTG |
CTTCCTCTTCCCTTCACACCCACACGCACACCTGGCCAACTTGTATGAGCGTGTGTGTGCTCGTTTTCGCGATTGTGCGTTCGTGTGGGTGCTTAACGCACACACTTACAGCTCGTTTTTATGCAATGTGTATGTAATTTGCAATGCACTTTTCCAGTGAAACTCACCAGAACTCCTGAACTTCCATCGCCAATCGGTGACGTTTGCTTATGAAAAATTAAGCCCTTTCCGACGAAGACCTCCCTTTTGTTTGTTTACTAACATCGACTAGCATCAACGTCCCAATAATAACATTTAAAACGGAAGTTTGTGCAGGCCGACAATTTGTTGCAAGCTTTTCCTGCATCTTCAGCTTCGAAGCTGCAGTGTGAACGTGGCTGCAAAATATACCAGCAACTTTCGGAAGAATACCAAGGGAAACAAGCTTTTCGCTTAAAAATACCATTTGGCGCCAATTAACAACGGTATTTAAATATTTAAATATGAAACTCATATGAATCAATTTTCATTATTTTACTTTGGCTAGACAATCTGAGTAAAAATCAACACAAGTCGAAACAAACACAAACAAACAATTAAATACTATTTATATTTGATCCTTAGTTTAATCACATCTTAAATGTGATACATTTTATCCAAGCATATCGTTATGACCTGCATATAGCGATCCCAAAGACATCTGGTTTCTTGGTCCCTGCAAAACATTTAGAGTTGATTATCAAAAAAGTTACTTGATTAATCTAGACAAAAGTACCCACCTTGATATTTCCAGATCCAAAACGCTTTGCGTTATGGAAGCCAAAAATTTATTCACTTGCTCACGGTTCCAGCCATTCTGACGCCAAGGATCAATAAGCTTCTCACAGAGATCGACGAAAAGATCGCGCAGCTCCCGATTGCGATGCAAATTGCAGGCGATGGTGATCAGGGCGCGCCAGTAAGAACTGCAAATCAAAACAGATCAATAGTAAGAAAAGGTGATAATGTCGAGAAATAAGTCCTTACCGAAAGTTGCCATCCAGTTCGTGAAAGGCGCGCTCGAGAAGAACGGGTTTGAGGCGCATGCAGACGAGGCTAAATGCAAGGACTAAACTATATAATAGCTCGTGTGCGGTAGAAAAATAAATCTCACTGCTTAATCTCCTTTTCCTTGTCGAGTAGACAGCGCAACTCCCGCAGGTCCAAGAGAAACT |
Search Again
