| Donor | Location | Cytology | FBti | Stock number |
|---|---|---|---|---|
| gRNA_int200_NR2E1_IOH_R46W | 3R:30852315..30854400 | 100A6-100A | Order from Bellen Lab |
PCR check relative to sgRNA sequence
| HA-L | HA-R | Forward | Reverse |
|---|---|---|---|
| OK | OK | L-F AGTCCACATCGGAGTAACCAAGGA L-R ttgcaggggatatctaaaatgcg | R-R CCGACCTCAAAGCACTTCC R-F cgcatgattatctttaacgtacgtcac |
| sgRNA sequence | |
| sgRNA2 sequence |
| left_200bp | right_200bp |
|---|---|